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mouse neuronal cell line pc12  (Procell Inc)

 
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    Procell Inc mouse neuronal cell line pc12
    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in <t>PC12</t> cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Mouse Neuronal Cell Line Pc12, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+neuronal+cell+line+pc12/pc12+cells/pmc10958405-34-8-15
    Average 90 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis"

    Article Title: Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.14689

    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Figure Legend Snippet: Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Techniques Used: Activation Assay, Western Blot, Expressing, Control, Flow Cytometry, Membrane, Gene Expression

    Related Articles

    Cell Culture:

    Article Title: Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis
    Article Snippet: .. The mouse microglial cell line BV2 and the mouse neuronal cell line PC12, purchased from Procell Life, China, were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Excellbio, China) and 1% penicillin and streptomycin (Gibco, USA) at 37°C and 5% CO2. .. According to the manufacturer's instructions, BV2 cells were transfected with negative control‐siRNA (siNC) or STING‐siRNA (siSTING) (IGEBio Co., Ltd, Guangzhou, China) by Lipofectamine RNAiMAX (Thermo Fisher, USA).



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    mouse neuronal cell line pc12  (ATCC)
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    Figure 5. Cross-reactive of GDH from various rat tissues with hGDH mAbs (hGDH60-6) (A): lane 1, liver; lane 2, testis; lane 3, brain; lane 4, heart; lane 5, lung; lane 6, stomach; lane 7, muscle. Total proteins of the rat tissue homogenates were immunoblotted with the hGDH mAb. (B) An immunoblot of total proteins of various mammalians cell lines. Lane 1, mouse pheochromocytoma (adrenal gland) <t>PC12;</t> lane 2, mouse macrophage J774A1; lane 3, human hepatocellular car- cinoma (liver) HepG2; lane 4, human squamous cell carcinoma (cervix) SiHa; lane 5, human adenocarcinoma (cervix) HL3T1. The cells were grown in DME containing 10% FBS and total proteins were extracted and immunoblotted.
    Mouse Neuronal Cell Line Pc12, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+neuronal+cell+line+pc12/PC-12/pm14508063-100-0-12
    Average 98 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    mouse neuronal cell line pc12  (Procell Inc)
    90
    Procell Inc mouse neuronal cell line pc12
    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in <t>PC12</t> cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Mouse Neuronal Cell Line Pc12, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+neuronal+cell+line+pc12/pc12+cells/pmc10958405-34-8-15
    Average 90 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5. Cross-reactive of GDH from various rat tissues with hGDH mAbs (hGDH60-6) (A): lane 1, liver; lane 2, testis; lane 3, brain; lane 4, heart; lane 5, lung; lane 6, stomach; lane 7, muscle. Total proteins of the rat tissue homogenates were immunoblotted with the hGDH mAb. (B) An immunoblot of total proteins of various mammalians cell lines. Lane 1, mouse pheochromocytoma (adrenal gland) PC12; lane 2, mouse macrophage J774A1; lane 3, human hepatocellular car- cinoma (liver) HepG2; lane 4, human squamous cell carcinoma (cervix) SiHa; lane 5, human adenocarcinoma (cervix) HL3T1. The cells were grown in DME containing 10% FBS and total proteins were extracted and immunoblotted.

    Journal: Experimental & molecular medicine

    Article Title: Human glutamate dehydrogenase is immunologically distinct from other mammalian orthologues.

    doi: 10.1038/emm.2003.33

    Figure Lengend Snippet: Figure 5. Cross-reactive of GDH from various rat tissues with hGDH mAbs (hGDH60-6) (A): lane 1, liver; lane 2, testis; lane 3, brain; lane 4, heart; lane 5, lung; lane 6, stomach; lane 7, muscle. Total proteins of the rat tissue homogenates were immunoblotted with the hGDH mAb. (B) An immunoblot of total proteins of various mammalians cell lines. Lane 1, mouse pheochromocytoma (adrenal gland) PC12; lane 2, mouse macrophage J774A1; lane 3, human hepatocellular car- cinoma (liver) HepG2; lane 4, human squamous cell carcinoma (cervix) SiHa; lane 5, human adenocarcinoma (cervix) HL3T1. The cells were grown in DME containing 10% FBS and total proteins were extracted and immunoblotted.

    Article Snippet: Mouse neuronal cell line PC12, mouse macrophage J774A1, human hepatocellular carcinoma HepG2 (ATCC No. HB-8065), human squamous cell carcinoma SiHa (ATCC No. HTB17), and human cervical adenocarcinoma HL3T1 were obtained from Korean Cell Line Bank.

    Techniques: Western Blot

    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis

    doi: 10.1111/cns.14689

    Figure Lengend Snippet: Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Article Snippet: The mouse microglial cell line BV2 and the mouse neuronal cell line PC12, purchased from Procell Life, China, were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Excellbio, China) and 1% penicillin and streptomycin (Gibco, USA) at 37°C and 5% CO2.

    Techniques: Activation Assay, Western Blot, Expressing, Control, Flow Cytometry, Membrane, Gene Expression